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cd44 positive selection  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd44 positive selection
    Cd44 Positive Selection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd44+positive+selection/CD44+MicroBeads%2C+human/10__32592_slash_ircmj__2023__25__3__2398-65-3-22
    Average 94 stars, based on 115 article reviews
    cd44 positive selection - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Selection:

    Article Title: Overexpression of Argonaute HIWI Gene in Colorectal Cancer Stem Cells and Colorectal Cancerous Tissue
    Article Snippet: HT-29 cell culture The human colorectal adenocarcinoma cell line HT-29 (ATCC® HTB-38TM) was cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 100 μg/ml streptomycin, 100 μg/ml penicillin in a humidi ed incubator containing 5% CO2 at 37 ̊C. .. Cell separation by CD44 positive selection The human colorectal adenocarcinoma cell line HT-29 was passed through a MACS column [Order number: 130-095-194, Miltenyi Biotec, UK], including CD44 monoclonal antibody, thereby CD44+ HT29 cells were separated from other cells, and then they were cultured. ..

    Article Title: Overexpression of Argonaute HIWI Gene in Colorectal Cancer Stem Cells and Colorectal Cancerous Tissue
    Article Snippet: The human colorectal adenocarcinoma cell line HT-29 (ATCC® HTB-38TM) was cultured in RPMI1640 medium supplemented with 10% fetal bovine serum (FBS), 100 μg/ml streptomycin, and 100 μg/ml penicillin in a humidified incubator containing 5% CO2 at 37°C. .. 3.3.Cell separation by CD44 positive selection The human colorectal adenocarcinoma cell line HT-29 was passed through a MACS column [Order number: 130-095-194, Miltenyi Biotec, UK], including CD44 monoclonal antibody, thereby CD44+ HT29 cells were separated from other cells, and then they were cultured. ..

    Article Title: Small cell lung cancer stem cells display mesenchymal properties and exploit immune checkpoint pathways in activated cytotoxic T lymphocytes.
    Article Snippet: .. 1 3 H69Sc cells were isolated from the H69Adh subpopulation through CD44 positive selection by magnetic-activated cell sorting (MACS) (Miltenyi) which was followed by fluorescence-activated cell sorting (FACS) of CD44+CD90+ cells. .. For certain experiments, SCLC cells were treated with 150 ng/mL recombinant IFN-γ (R&D Biosystems) for 24 or 48 h. Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors’ blood samples (Hacettepe University Local Ethics Committee, approval no. GO 17/503) by density gradient centrifugation (Ficoll 1.077; GE Healthcare).

    Article Title: Small cell lung cancer stem cells display mesenchymal properties and exploit immune checkpoint pathways in activated cytotoxic T lymphocytes
    Article Snippet: .. H69Sc cells were isolated from the H69Adh subpopulation through CD44 positive selection by magnetic-activated cell sorting (MACS) (Miltenyi) which was followed by fluorescence-activated cell sorting (FACS) of CD44 + CD90 + cells. .. For certain experiments, SCLC cells were treated with 150 ng/mL recombinant IFN-γ (R&D Biosystems) for 24 or 48 h. Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors’ blood samples (Hacettepe University Local Ethics Committee, approval no. GO 17/503) by density gradient centrifugation (Ficoll 1.077; GE Healthcare).

    Magnetic Cell Separation:

    Article Title: Overexpression of Argonaute HIWI Gene in Colorectal Cancer Stem Cells and Colorectal Cancerous Tissue
    Article Snippet: HT-29 cell culture The human colorectal adenocarcinoma cell line HT-29 (ATCC® HTB-38TM) was cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 100 μg/ml streptomycin, 100 μg/ml penicillin in a humidi ed incubator containing 5% CO2 at 37 ̊C. .. Cell separation by CD44 positive selection The human colorectal adenocarcinoma cell line HT-29 was passed through a MACS column [Order number: 130-095-194, Miltenyi Biotec, UK], including CD44 monoclonal antibody, thereby CD44+ HT29 cells were separated from other cells, and then they were cultured. ..

    Article Title: Overexpression of Argonaute HIWI Gene in Colorectal Cancer Stem Cells and Colorectal Cancerous Tissue
    Article Snippet: The human colorectal adenocarcinoma cell line HT-29 (ATCC® HTB-38TM) was cultured in RPMI1640 medium supplemented with 10% fetal bovine serum (FBS), 100 μg/ml streptomycin, and 100 μg/ml penicillin in a humidified incubator containing 5% CO2 at 37°C. .. 3.3.Cell separation by CD44 positive selection The human colorectal adenocarcinoma cell line HT-29 was passed through a MACS column [Order number: 130-095-194, Miltenyi Biotec, UK], including CD44 monoclonal antibody, thereby CD44+ HT29 cells were separated from other cells, and then they were cultured. ..

    Article Title: Small cell lung cancer stem cells display mesenchymal properties and exploit immune checkpoint pathways in activated cytotoxic T lymphocytes.
    Article Snippet: .. 1 3 H69Sc cells were isolated from the H69Adh subpopulation through CD44 positive selection by magnetic-activated cell sorting (MACS) (Miltenyi) which was followed by fluorescence-activated cell sorting (FACS) of CD44+CD90+ cells. .. For certain experiments, SCLC cells were treated with 150 ng/mL recombinant IFN-γ (R&D Biosystems) for 24 or 48 h. Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors’ blood samples (Hacettepe University Local Ethics Committee, approval no. GO 17/503) by density gradient centrifugation (Ficoll 1.077; GE Healthcare).

    Article Title: Small cell lung cancer stem cells display mesenchymal properties and exploit immune checkpoint pathways in activated cytotoxic T lymphocytes
    Article Snippet: .. H69Sc cells were isolated from the H69Adh subpopulation through CD44 positive selection by magnetic-activated cell sorting (MACS) (Miltenyi) which was followed by fluorescence-activated cell sorting (FACS) of CD44 + CD90 + cells. .. For certain experiments, SCLC cells were treated with 150 ng/mL recombinant IFN-γ (R&D Biosystems) for 24 or 48 h. Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors’ blood samples (Hacettepe University Local Ethics Committee, approval no. GO 17/503) by density gradient centrifugation (Ficoll 1.077; GE Healthcare).

    Cell Culture:

    Article Title: Overexpression of Argonaute HIWI Gene in Colorectal Cancer Stem Cells and Colorectal Cancerous Tissue
    Article Snippet: HT-29 cell culture The human colorectal adenocarcinoma cell line HT-29 (ATCC® HTB-38TM) was cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 100 μg/ml streptomycin, 100 μg/ml penicillin in a humidi ed incubator containing 5% CO2 at 37 ̊C. .. Cell separation by CD44 positive selection The human colorectal adenocarcinoma cell line HT-29 was passed through a MACS column [Order number: 130-095-194, Miltenyi Biotec, UK], including CD44 monoclonal antibody, thereby CD44+ HT29 cells were separated from other cells, and then they were cultured. ..

    Article Title: Overexpression of Argonaute HIWI Gene in Colorectal Cancer Stem Cells and Colorectal Cancerous Tissue
    Article Snippet: The human colorectal adenocarcinoma cell line HT-29 (ATCC® HTB-38TM) was cultured in RPMI1640 medium supplemented with 10% fetal bovine serum (FBS), 100 μg/ml streptomycin, and 100 μg/ml penicillin in a humidified incubator containing 5% CO2 at 37°C. .. 3.3.Cell separation by CD44 positive selection The human colorectal adenocarcinoma cell line HT-29 was passed through a MACS column [Order number: 130-095-194, Miltenyi Biotec, UK], including CD44 monoclonal antibody, thereby CD44+ HT29 cells were separated from other cells, and then they were cultured. ..

    Isolation:

    Article Title: Small cell lung cancer stem cells display mesenchymal properties and exploit immune checkpoint pathways in activated cytotoxic T lymphocytes.
    Article Snippet: .. 1 3 H69Sc cells were isolated from the H69Adh subpopulation through CD44 positive selection by magnetic-activated cell sorting (MACS) (Miltenyi) which was followed by fluorescence-activated cell sorting (FACS) of CD44+CD90+ cells. .. For certain experiments, SCLC cells were treated with 150 ng/mL recombinant IFN-γ (R&D Biosystems) for 24 or 48 h. Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors’ blood samples (Hacettepe University Local Ethics Committee, approval no. GO 17/503) by density gradient centrifugation (Ficoll 1.077; GE Healthcare).

    Article Title: Small cell lung cancer stem cells display mesenchymal properties and exploit immune checkpoint pathways in activated cytotoxic T lymphocytes
    Article Snippet: .. H69Sc cells were isolated from the H69Adh subpopulation through CD44 positive selection by magnetic-activated cell sorting (MACS) (Miltenyi) which was followed by fluorescence-activated cell sorting (FACS) of CD44 + CD90 + cells. .. For certain experiments, SCLC cells were treated with 150 ng/mL recombinant IFN-γ (R&D Biosystems) for 24 or 48 h. Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors’ blood samples (Hacettepe University Local Ethics Committee, approval no. GO 17/503) by density gradient centrifugation (Ficoll 1.077; GE Healthcare).

    FACS:

    Article Title: Small cell lung cancer stem cells display mesenchymal properties and exploit immune checkpoint pathways in activated cytotoxic T lymphocytes.
    Article Snippet: .. 1 3 H69Sc cells were isolated from the H69Adh subpopulation through CD44 positive selection by magnetic-activated cell sorting (MACS) (Miltenyi) which was followed by fluorescence-activated cell sorting (FACS) of CD44+CD90+ cells. .. For certain experiments, SCLC cells were treated with 150 ng/mL recombinant IFN-γ (R&D Biosystems) for 24 or 48 h. Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors’ blood samples (Hacettepe University Local Ethics Committee, approval no. GO 17/503) by density gradient centrifugation (Ficoll 1.077; GE Healthcare).

    Article Title: Small cell lung cancer stem cells display mesenchymal properties and exploit immune checkpoint pathways in activated cytotoxic T lymphocytes
    Article Snippet: .. H69Sc cells were isolated from the H69Adh subpopulation through CD44 positive selection by magnetic-activated cell sorting (MACS) (Miltenyi) which was followed by fluorescence-activated cell sorting (FACS) of CD44 + CD90 + cells. .. For certain experiments, SCLC cells were treated with 150 ng/mL recombinant IFN-γ (R&D Biosystems) for 24 or 48 h. Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors’ blood samples (Hacettepe University Local Ethics Committee, approval no. GO 17/503) by density gradient centrifugation (Ficoll 1.077; GE Healthcare).

    Fluorescence:

    Article Title: Small cell lung cancer stem cells display mesenchymal properties and exploit immune checkpoint pathways in activated cytotoxic T lymphocytes.
    Article Snippet: .. 1 3 H69Sc cells were isolated from the H69Adh subpopulation through CD44 positive selection by magnetic-activated cell sorting (MACS) (Miltenyi) which was followed by fluorescence-activated cell sorting (FACS) of CD44+CD90+ cells. .. For certain experiments, SCLC cells were treated with 150 ng/mL recombinant IFN-γ (R&D Biosystems) for 24 or 48 h. Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors’ blood samples (Hacettepe University Local Ethics Committee, approval no. GO 17/503) by density gradient centrifugation (Ficoll 1.077; GE Healthcare).

    Article Title: Small cell lung cancer stem cells display mesenchymal properties and exploit immune checkpoint pathways in activated cytotoxic T lymphocytes
    Article Snippet: .. H69Sc cells were isolated from the H69Adh subpopulation through CD44 positive selection by magnetic-activated cell sorting (MACS) (Miltenyi) which was followed by fluorescence-activated cell sorting (FACS) of CD44 + CD90 + cells. .. For certain experiments, SCLC cells were treated with 150 ng/mL recombinant IFN-γ (R&D Biosystems) for 24 or 48 h. Peripheral blood mononuclear cells (PBMC) were isolated from healthy donors’ blood samples (Hacettepe University Local Ethics Committee, approval no. GO 17/503) by density gradient centrifugation (Ficoll 1.077; GE Healthcare).



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